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In-Stock AAV Vectors – Dr. Karl Deisseroth, 100ul Aliquots

We are pleased to stock optogenetic constructs from at Stanford University. For first time orders of these viruses, Laboratory PIs please contact Dr. Karl Deisseroth (deissero@stanford.edu) and Charu Ramakrishnan (charur@stanford.edu) for an MTA.Please upload a copy of your MTA when placing your online order.

The following constructs are available in AAV2 and AAV5 with the following exceptions: GFAP constructs available in AAV1, 2, 5 & 8, AAV-EF1a-mCherry-IRES-WGA-Cre available in AAV2, 5 & 8, C1V1(t/t) and SwiChRCA in AAV2, 5 and DJ, 20ul concentrated high titer AAV-hSyn-hChR2(H134R)-EYFP (≥1x10E13 vg/ml) available in AAV6 and Opto-XRs in AAV5. For other serotypes, please place an order for a custom prep.

In Stock AAV Price per 100ul

• External Academic: $220
• ºÚÁÏÍø Investigator: $150
• Corporate: $340

Optical Excitation: ChR2

• AAV-CaMKIIa-hChR2(H134R)-EYFP
• AAV-CaMKIIa-hChR2(H134R)-mCherry
• AAV-hSyn-hChR2(H134R)-EYFP
• AAV-hSyn-hChR2(H134R)-mCherry
• 20ul – High Titer AAV-hSyn-hChR2(H134R)-EYFP (AAV6 only)

Cre-inducible AAV: DIO-AAV

• AAV-EF1a-DIO-hChR2(H134R)-EYFP
• AAV-EF1a-DIO-hChR2(H134R)-mCherry

Third-generation Optogenetic Inhibition: eNpHR3.0

• AAV-EF1a-DIO-eNpHR3.0-EYFP
• AAV-EF1a-DIO-eNpHR3.0-mCherry
• AAV-CaMKIIa-eNpHR3.0-EYFP
• AAV-CaMKIIa-eNpHR3.0-mCherry
• AAV-hSyn-eNpHR3.0-EYFP
• AAV-hSyn-eNpHR3.0-mCherry
• AAV-hThy1-eNpHR3.0-EYFP

Red-shifted Optical Excitation: C1V1 Variants

• AAV-CaMKIIa-C1V1(E122T/E162T)-TS-EYFP
• AAV-CaMKIIa-C1V1(E122T/E162T)-TS-mCherry
• AAV-EF1a-DIO-C1V1(E122T/E162T)-TS-EYFP
• AAV-EF1a-DIO-C1V1(E122T/E162T)-TS-mCherry
• AAV-CaMKIIa-C1V1(E162T)-p2A-EYFP

C1V1(t/t) (AAV2, 5, DJ)

• AAV-hSyn-C1V1(t/t)-TS-EYFP
• AAV-hSyn-C1V1(t/t)-TS-p2A-EYFP

Stabilized Step Function Opsins: SSFOs

• AAV-CaMKIIa-hChR2(C128S/D156A)-EYFP
• AAV-CaMKIIa-hChR2(C128S/D156A)-mCherry
• AAV-EF1a-DIO-hChR2(C128S/D156A)-EYFP
• AAV-EF1a-DIO-hChR2(C128S/D156A)-mCherry

Second-generation Ultrafast Optogenetic Control

• AAV-CaMKIIa-hChR2(E123T/T159C)-EYFP
• AAV-CaMKIIa-hChR2(E123T/T159C)-mCherry
• AAV-CaMKII-hChR2(E123A)-EYFP
• AAV-CaMKII-hChR2(E123A)-mCherry
• AAV-EF1a-DIO-hChR2(E123T/T159C)-EYFP
• AAV-EF1a-DIO-hChR2(E123T/T159C)-mCherry
• AAV-EF1a-DIO-ChR2(T159C)-EYFP
• AAV-EF1a-DIO-ChR2(T159C)-mCherry
• AAV-EF1a-DIO-ChR2(E123A)-EYFP
• AAV-EF1a-DIO-ChR2(E123A)-mCherry
• AAV-hSyn-ChR2(E123A)-EYFP

Third-generation Optogenetic Inhibition: Arch 3.0 & ArchT 3.0

• AAV-CaMKIIa-eArch3.0-EYFP
• AAV-hSyn-eArch3.0-EYFP
• AAV-EF1a-DIO-eArch3.0-EYFP
• AAV-CaMKIIa-eArchT3.0-EYFP

Transsynaptic Tracers: WGA-Cre (AAV2, 5 & 8)

• AAV-EF1a-mCherry-IRES-WGA-Cre

Ultrafast Optogenetic Control: ChETA

• AAV-EF1a-DIO-ChETA-EYFP

Cell Filling Variants

• AAV-CaMKII-hChR2(T159C)-p2A-EYFP-WPRE
• AAV-CaMKII-hChR2(T159C)-p2A-mCherry-WPRE
• AAV-CaMKII-hChR2(E123T/T159C)-p2A-EYFP-WPRE
• AAV-CaMKII-hChR2(E123T/T159C)-p2A-mCherry-WPRE
• AAV-CaMKIIa-C1V1(E162T)-TS-p2A-EYFP-WPRE
• AAV-CaMKIIa-C1V1(E162T)-TS-p2A-mCherry-WPRE
• AAV-CaMKIIa-C1V1(t/t)-TS-p2A-EYFP-WPRE
• AAV-CaMKIIa-C1V1(t/t)-TS-p2A-mCherry-WPRE
• AAV-EF1a-DIO-hChR2(E123T/T159C)-p2A-EYFP-WPRE
• AAV-EF1a-DIO-hChR2(E123T/T159C)-p2A-mCherry-WPRE
• AAV-EF1a-DIO-C1V1(E162T)-TS-p2A-EYFP-WPRE
• AAV-EF1a-DIO-C1V1(E162T)-TS-p2A-mCherry-WPRE
• AAV-EF1a-DIO-C1V1(t/t)-TS-p2A-EYFP-WPRE
• AAV-EF1a-DIO-C1V1(t/t)-TS-p2A-mCherry-WPRE

SwiChRCA (AAV2, 5, DJ)

• AAV-CaMKIIa-SwiChRCA-TS-EYFP
• AAV-EF1a-DIO-SwiChRCA-TS-EYFP-WPRE
• AAV-hSyn-SwiChRCA-TS-EYFP

Control Fluorophores

• AAV-CaMKIIa-EYFP
• AAV-CaMKIIa-mCherry
• AAV-EF1a-DIO-EYFP
• AAV-EF1a-DIO-mCherry
• AAV-hSyn-EYFP
• AAV-hSyn-mCherry

Optical Excitation: ChR2-GFAP (AAV1, 2, 5 & 8)

• AAV-GFAP-hChR2(H134R)-EYFP
• AAV-GFAP-hChR2(H134R)-mCherry