ĐĎॹá>ţ˙ VXţ˙˙˙U˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙˙ěĽÁ‰a đż*(jbjbYQYQ $@3333*"˙˙˙˙˙˙ˆhhhhhhh|ÄBÄBÄBÄB ĐBL|ąC"(C(C(C(C(C(C(C(C(C*C*C*C*C*C*C,ÓDR%G”VCh(C(C(C(C(CVC(Chh(C(CkC(C(C(C(Ch(Ch(C(C(C||hhhh(C(C(C(Chh(CC ĄĘ2ÂÄB(C(C(CC0ąC(CšG(CšG(C(Ch||¤ "¤ || "pLIC-MBP vector 6423bp. SspI site at 6204. 6xHis-MBP translation start at 4999. TEV site at 6181. parent vector is pET28/30. 1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg 51 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct 101 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg 151 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac 201 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acattaacgc 251 ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt 301 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa 351 ccctgataaa tgcttcaata atttgaaaaa ggaagagtat gagtattcaa 401 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt 451 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt 501 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc 551 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa 601 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc 651 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca 701 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg 751 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga 801 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg 851 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat 901 accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt 951 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa 1001 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc 1051 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc 1101 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc 1151 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg 1201 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac 1251 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat 1301 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac 1351 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag 1401 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc 1451 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 1501 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc 1551 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc 1601 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc 1651 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa 1701 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg 1751 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 1801 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg 1851 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag 1901 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca 1951 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc 2001 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc 2051 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga 2101 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa 2151 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg 2201 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg 2251 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac 2301 actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc 2351 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt 2401 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca 2451 ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg 2501 gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt 2551 tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg 2601 ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg 2651 atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca 2701 cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag 2751 ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca 2801 gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta 2851 gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct 2901 gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca 2951 tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc 3001 gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag 3051 cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggggc 3101 cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg 3151 gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca 3201 agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa 3251 aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga 3301 agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag 3351 gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc 3401 ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt 3451 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc 3501 gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca 3551 ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag 3601 agttgcagca agcggtccac gctggtttgc cccagcaggc gaaaatcctg 3651 tttgatggtg gttaacggcg ggatataaca tgagctgtct tcggtatcgt 3701 cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta 3751 atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc 3801 agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg 3851 acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg 3901 cgagtgagat atttatgcca gccagccaga cgcagacgcg ccgagacaga 3951 acttaatggg cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca 4001 gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg 4051 ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt 4101 gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa 4151 tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta 4201 caggcttcga cgccgcttcg ttctaccatc gacaccacca cgctggcacc 4251 cagttgatcg gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt 4301 gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc 4351 gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc 4401 cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca 4451 ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg 4501 tataacgtta ctggtttcac attcaccacc ctgaattgac tctcttccgg 4551 gcgctatcat gccataccgc gaaaggtttt gcgccattcg atggtgtccg 4601 ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag 4651 tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca 4701 aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc 4751 acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc 4801 atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg 4851 tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat cgatctcgat 4901 cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa 4951 ttcccctcta gaaataattt tgtttaactt taagaaggag atatacatat 5001 gaaacaccat caccatcacc atggcaaaat cgaagaaggt aaactggtaa 5051 tctggattaa cggcgataaa ggctataacg gtctcgctga agtcggtaag 5101 aaattcgaga aagataccgg aattaaagtc accgttgagc atccggataa 5151 actggaagag aaattcccac aggttgcggc aactggcgat ggccctgaca 5201 ttatcttctg ggcacacgac cgctttggtg gctacgctca atctggcctg 5251 ttggctgaaa tcaccccgga caaagcgttc caggacaagc tgtatccgtt 5301 tacctgggat gccgtacgtt acaacggcaa gctgattgct tacccgatcg 5351 ctgttgaagc gttatcgctg atttataaca aagatctgct gccgaacccg 5401 ccaaaaacct gggaagagat cccggcgctg gataaagaac tgaaagcgaa 5451 aggtaagagc gcgctgatgt tcaacctgca agaaccgtac ttcacctggc 5501 cgctgattgc tgctgacggg ggttatgcgt tcaagtatga aaacggcaag 5551 tacgacatta aagacgtggg cgtggataac gctggcgcga aagcgggtct 5601 gaccttcctg gttgacctga ttaaaaacaa acacatgaat gcagacaccg 5651 attactccat cgcagaagct gcctttaata aaggcgaaac agcgatgacc 5701 atcaacggcc cgtgggcatg gtccaacatc gacaccagca aagtgaatta 5751 tggtgtaacg gtactgccga ccttcaaggg tcaaccatcc aaaccgttcg 5801 ttggcgtgct gagcgcaggt attaacgccg ccagtccgaa caaagagctg 5851 gcaaaagagt tcctcgaaaa ctatctgctg actgatgaag gtctggaagc 5901 ggttaataaa gacaaaccgc tgggtgccgt agcgctgaag tcttacgagg 5951 aagagttggc gaaagatcca cgtattgccg ccaccatgga aaacgcccag 6001 aaaggtgaaa tcatgccgaa catcccgcag atgtccgctt tctggtatgc 6051 cgtgcgtact gcggtgatca acgccgccag cggtcgtcag actgtcgatg 6101 aagccctgaa agacgcgcag actaattcga gctcgaacaa caacaacaat 6151 aacaataaca acaacagaga tctgggtacc gagaacctgt acttccaatc 6201 caatattgga agtggataac ggatccgaat tcgagctccg tcgacaagct 6251 tgcggccgca ctcgagcacc accaccacca ccactgagat ccggctgcta 6301 acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa 6351 ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct 6401 gaaaggagga actatatccg gat *(÷hŹ9ÚhŹ9ÚPJ‚ƒ„ĆÇ  LMŇÓXY›œŢß! 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