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Workbook wSummaryInformation( DocumentSummaryInformation8+ =\pcholly Vanhooke Ba==a88@"1HVerdana1HVerdana1HVerdana1HVerdana1HVerdana1HSymbol1 HVerdana1=HVerdana1HVerdana"$"#,##0_);\("$"#,##0\)!"$"#,##0_);[Red]\("$"#,##0\)""$"#,##0.00_);\("$"#,##0.00\)'""$"#,##0.00_);[Red]\("$"#,##0.00\)7*2_("$"* #,##0_);_("$"* \(#,##0\);_("$"* "-"_);_(@_).))_(* #,##0_);_(* \(#,##0\);_(* "-"_);_(@_)?,:_("$"* #,##0.00_);_("$"* \(#,##0.00\);_("$"* "-"??_);_(@_)6+1_(* #,##0.00_);_(* \(#,##0.00\);_(* "-"??_);_(@_)                + ) , *    "          8dq:Fc-2NWgFSWc-2NWgFSW̙̙3f3fff3f3f33333f33333\`JSheet1:8Sheet2WSheet3߮##5' anneal sitef wfprimes at = position on vector where primer starts; 5' anneal site = position on vector where 5' end of the lic insertThe first three primers listed above sit further upstream than sequencing primers currently used by the Sondek lab (see note f). 4All are available from Janeen in the Protein Core. anneals to the vector primer name useful forGTT GTA TGA CGC TCT TGA TGT TGpLIC-GST pGST-LIC2 pFB-GST-LICTCC CGC GAA ATT AAT ACG ACT CACpLIC-Hisp15-LICp15-LIC2p15strepHT-LICTCG ATG AAG CCC TGA AAG ACpLIC-MBPCTT TCA TAA TTG CGA CTG G pPICZ A,B,C pPICZa A,B,C pPICZb_LICsequence (5' to 3') Protein Core Sequencing Primersa p15hislic seqb gsttevc mbplicd ppiczb 842fore NotescPrimes at the 3' end of GSTdPrimes at the 3' end of MBPuaThese primers are designed to ensure that the front end of the LIC-cloned product is obtained in the sequencing run.AePrimes upstream of the 5' AOX1 sequencing primer from Invitrogen+bPrimes upstream of the T7 promoter primer  primes atf " A O +q H #,,,( e edyK##J`P HiL, ,,,,0HiL` ,2vRpU4SxN3O ` , pOe>OȝH` , ,TA^0HHրUOZZ U HHHրUOZ0OZ$@S`@|\U$`Z$HOO@4@K0HOS$HjJ`PHȵN2 H̿ [ ,R\c[a`( N3 J`P HiL, ,`( e J( , ,p'^0U,, p۸0ȗpHp,p N2p( N3 = $/67  dMbP?_*+%&?'?(?)?M com.apple.print.PageFormat.PMHorizontalRes com.apple.print.ticket.creator com.apple.printingmanager com.apple.print.ticket.itemArray com.apple.print.PageFormat.PMHorizontalRes 300 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com.apple.print.PaperInfoTicket com.apple.print.ticket.APIVersion 00.20 com.apple.print.ticket.privateLock com.apple.print.ticket.type com.apple.print.PageFormatTicket Mz,, ` g(HH(dh "d??U >@dd@    !"#$%&'()*,-./1 Oh+'0x HPh 'Janeen VanhookeJaneen VanhookeMicrosoft Excel@q,z@zG PICT | HH| |    ""*"7"F"S"b"o"|"""""")"6"E"R"a"n"{"""""""+"8"G"T"c"p"}"""""","9"H"U"d"q"~"""     " "k " " "j " " " "l " " "m 1"" "" " " "" DDQQ^^, Lucida Grande .+ 1 ** 2 ,7* 3 |*4 HS* 5 |*6 do* 7 q|* 8 ~* 9 (10 * 11 |*12( MA)B)C |, #Verdana# (D primer name)sequence (5' to 3')) useful for Tb|(` p15hislic seq ([eb +%TCC CGC GAA ATT AAT ACG ACT CAC)pLIC-His |* p15-LIC* p15-LIC2* p15strepHT-LIC |(gsttev (?c +KGTT GTA TGA CGC TCT TGA TGT TG)pLIC-GST k(Protein Core Sequencing Primers (a |"   kk    ** 77 FF SS bb oo ||     {  ՜.+,0 PXd lt| ' Sheet1Sheet2Sheet3  WorksheetsCompObj0X FMicrosoft Excel Worksheet8FIBExcel.Sheet.8