ࡱ> <>;5@ 8'bjbj22 (0XXJ2222 L. NV V V V V E E E $RVlEE  @E E E 22V V E 42RV V E "V J 6S& y 0N j2222E E E E E E E ~ d ~ ZYM-5052 Media (F. William Studier) N-Z-amine AS 1% 10g Yeast extract 0.5% 5g Na2HPO4 25mM KH2PO4 25mM 50xM 20ml NH4Cl 50mM Na2SO4 5mM Mg SO4 2mM 1M 2ml Trace Metals* 0.2x 1000x 0.2ml Glycerol 0.5% Glucose 0.05% 50x 5052 20ml a-D-Lactose 0.2% Stock per 1L Add sterilized stocks to autoclaved ZY (combined volume - 42.2ml to 958ml of media) right before use. Stock Solutions 50x M MW stock g/800mL Na2HPO4x7 H20 268.07 1.25M 268.07 KH2PO4 136.09 1.25M 136.09 NH4Cl 53.49 2.5M 106.98 Na2SO4 142.04 0.25M 28.408 Weigh out salts in dry bottle, add water to less than 500ml, sterilize 15min and correct the volume with autoclaved water, store at room temperature. The solution is close to saturation. If crystals appear, heat in microwave to dissolve them. Mg SO4x7 H20 246.48 1M 123.24 50x 5052 stock /500ml Glycerol 25% 125ml Glucose 2.5% 12.5g a-D-Lactose 10% 50g 1000x Trace Metals* MW stock g/50mL stock/100ml 50mM FeCl3 162.21 0.1M 0.811 50ml 20mM CaCl2(x2 H20) 147.02 2M 14.7 1ml 10mM MnCl2 (x4 H20) 197.91 2M 19.79 500ml 10mM ZnSO4 (x7H2O) 287.54 2M 28.75 500ml 2mM Co$%.135>?@S\_`cdgjknoqryɯɣɣђɣɣɣɣɣɣɯɁ h"/h"/5B*CJ aJ phhCJ aJ h\lCJ H*aJ h\lh\lCJ H*aJ "jhCJ UaJ mHnHuhVCJ aJ h\lCJ aJ h\lh\lCJ aJ h\lCJ,aJ,hph5>*CJ,aJ,hph\l5>*CJ,aJ,2$%?]l $ N P  " B z   = > 5 6 gdwgd\l$a$gd\l('6'     $ 2 < @ N P   " $ B H J L T n p x z ~ ƾ}o}g[R[hK>5CJ$aJ$hph"/5CJ$aJ$h"/CJ$aJ$hphr5>*CJ$aJ$hphV5>*CJ$aJ$h vCJ$aJ$hwCJ$aJ$h"/hwCJ aJ hwCJ aJ hCJ$aJ$hVCJ$aJ$h&2CJ$aJ$hVhVCJ$aJ$hpCJ aJ h\lh\lCJ OJQJaJ h\lCJ aJ hCJ aJ hVCJ aJ ~       ! # $ % ( . 6 < = > j t 1 4 Ǽdz뢚ht#"CJ aJ hL~ CJ aJ hCJ aJ hCJ aJ h&2CJ aJ hK>CJ H*aJ h"/CJ H*aJ h"/hVCJ aJ hVCJ H*aJ hK>CJ aJ h"/CJ aJ h\lhVCJ aJ hVCJ aJ h\lhVCJ H*aJ 34 5 8 9 ; < B W X Y a e t u v 2468BD\^`ɻɐɈɀrdrSKh"/CJ$aJ$ h"/h_D05B*CJ aJ phhph5>*CJ$aJ$hph"/5>*CJ$aJ$h vCJ aJ hSCJ aJ h\lhrCJ OJQJaJ hrCJ$aJ$hpht#"5CJ$aJ$ht#"CJ$aJ$hphr5>*CJ$aJ$hrCJ aJ h"/CJ aJ h\lhVCJ H*aJ h\lhVCJ aJ hVCJ aJ hCJ aJ 6 X Y v 468`JH"""$#n###x&z&|& @ ^@ `gd"/p]^p`gdb=gdrgd\l`p   ļsk``T`khrhL~ CJ H*aJ h\lhL~ CJ aJ hL~ CJ aJ hrh"/CJ H*aJ hb=CJ aJ hV5CJ aJ h&2CJ aJ hCJ aJ hrCJ aJ hCJ H*aJ h"/h"/CJ H*aJ h"/CJ aJ h"/CJ$aJ$hr5CJ aJ h5CJ aJ hb=hb=5CJ aJ hb=h"/5CJ aJ hb=5CJ aJ ,46@H\^`bdfjlnr""""""""&"."0":"๥̹𹣹ԚhrhCJ H*aJ hCJ aJ hCJ H*aJ Uht#"CJ aJ hb=CJ OJQJaJ hCJ aJ h\lh"/CJ aJ hrCJ aJ hrhrCJ H*aJ hb=CJ aJ h&2CJ aJ h"/CJ aJ hL~ CJ aJ 4Cl2 (x6H2O) 237.93 2M 23.79 100ml 2mM CuCl2 134.44 2M 13.44 100ml 2mM NiCl2 129.62 1M 6.48 200ml 2mM Na2MoO4 205.92 1M 10.295 200ml 2mM Na2SeO3 172.94 2M 17.29 100ml 2mM H3BO3 61.83 200mM 0.62 1ml 0.1MFeCl3 stock resuspend in 100-fold diluted HCl (~0.12M HCl final), filter through 0.2mm filter and mix with autoclaved for 15min stocks of other metals to make the 1000x trace metals stock (46.3ml sterilized water plus stocks for 100 ml). Store at room temperature. Upon prolonged storage, small amounts of precipitate can be formed in the mixture. Next time 2mM NiCl2 129.62 200mM 1.30 1ml 2mM Na2MoO4 205.92 200mM 2.059 1ml } } :"<"B"D"F"X"Z"t"v""""""""""""""""""""""## ##### #"#0#2#8#>#@#L#V#^#`#b#h#j#l#n#x#z#~#####ܼܳܫܫhrhb=CJ H*aJ hXvCJ aJ hCJ aJ hCJ H*aJ hrhCJ H*aJ hwCJ aJ hrhrCJ H*aJ hCJ aJ hb=CJ OJQJaJ hb=CJ aJ h&2CJ aJ ;############<$@$d$f$~$$2%%%%v&x&z&|&&&&&&&"'(','.'2'4'̽赭~sodohhCJxaJxhhhCJaJhhCJ aJ hrhHHCJ H*aJ hHHCJ aJ h[8,CJ aJ hCJ aJ ht#"CJ aJ hCJ aJ hwhwCJ OJQJaJ hwCJ aJ hL~ CJ aJ hrhrCJ H*aJ hCJ aJ hb=CJ aJ hrCJ aJ %|&&&"'$'&'(','.'2'4'6'8'gdgdHHgd\l 4'6'8'hhCJ aJ hn6&1h:p&2/ =!"#$%@@@ NormalCJ_HaJmH sH tH DAD Default Paragraph FontRiR  Table Normal4 l4a (k(No ListR R0$%?]l 34 *IJABde  2["Hm"GHIJLMOS0@0p@000@0p@0@000000000000000 00000p00p0 0p00 0 0000 000000000 00 0000 0 0000000 00 %?]l *IASO900 $YM90Oy02J  tOy0O900 YO90O90O90R~ 4 ` :"#4'8' 6 |&8' 6' 8@f(  b  C "` b  C "` B S  ??R,<t, tJSJS 3 Je 2HJKMNSJSsvetal99L~ r&2pt#"[8,_D0K>HH\lXv vwn6SV5,"/b=V@IIAII@  RP@P P@PP"UnknownGz Times New Roman5Symbol3& z Arial"qh㚦FFV4 4 Yr4dGG 3H ?\lZYM-5052 Media (Fsvetal99svetal99Oh+'0 $ @ L X dpxZYM-5052 Media (FdYM- svetal99MedvetvetNormal9 svetal99Med16tMicrosoft Word 10.0@ @4Ҟ&@@ &4՜.+,0 hp  -CH GA ZYM-5052 Media (F Title  "#$%&'()*,-./012456789:=Root Entry F@k&?Data 1Table!WordDocument(0SummaryInformation(+DocumentSummaryInformation83CompObjj  FMicrosoft Word Document MSWordDocWord.Document.89q