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Camille Ehre, PhD

Associate Professor

Specialty Area:

Mucin Gene and Glycoprotein Expression Patterns

Research Focus:

Dr. Ehre鈥檚 laboratory specializes in the biophysical and biochemical properties of mucus, which are affected in various airway diseases such as cystic fibrosis (CF), chronic obstructive pulmonary disease (COPD) and primary ciliary dyskinesia (PCD). Studies conducted in her laboratory utilize cell cultures, human specimen and small animal models. Dr. Ehre also studies the infection pathway of SARS-CoV-2 and is investigating new therapies to block viral entry into airway cells.

Biophysical & Biochemical Properties of Mucus

Mucus is normally produced in the lungs to trap inhaled pathogens (e.g., bacteria, viruses) and particles (e.g., dust, smoke) but, in disease, increased mucus concentration leads to airflow obstruction, bacterial infection and chronic inflammation. Dr. Ehre鈥檚 research focuses on understanding the biological alterations of mucus that occur in disease and studying new pharmacological approaches to restore lung health.

The viscoelastic properties of mucus rely on the polymeric gel-forming mucins (mostly MUC5AC and MUC5B in the lungs), which are among the largest glycoproteins in the animal world. Working with mucus can be challenging because of the size of these mucin molecules and the complex ultrastructure and intermingled organization of mucin polymers. Dr. Ehre has established a series of assays to study mucin-mucin and mucin-DNA interactions based on confocal imaging, fluorescence-based quantification and multi-angle light scattering analysis, as well as techniques to measure changes in rheology.

Dr. Ehre also conducts preclinical studies testing compounds aimed at improving mucus clearance in the lungs. New pharmacological reagents are initially tested in vitro utilizing primary human bronchial epithelial cells (HBE) and/or human specimen (sputum or bronchoalveolar lavages). Subsequently, drug efficacy and toxicity are tested in vivo using mouse models of airway diseases such as the 尾ENaC model.

Confocal images exhibiting different mucin network topologies between CF and non-CF patients

Fig 1. Immunolabeling of mucins and DNA in pediatric bronchoalveolar lavages. Confocal images revealing different mucin network topologies between CF and non-CF patients. (Blue=DAPI, Green=MUC5B, Red=MUC5AC).

Figure containing AB-PAS stain and immunohistochemistry images, explained more fully in the figure legend

Fig 2. (A) Spontaneous formation of mucus plaques and neutrophilic inflammation in 尾ENaC mice, a mouse model mimicking the CF lung disease. (A) AB-PAS stain of 尾ENaC lungs revealing mucus plugs at airway branching. (B) Immunohistochemistry (IHC) displaying PCL collapse and mucin accumulation on airway surfaces (green=Muc5b, Blue=DAPI). (C) Inflammatory cells from a 尾ENaC mouse bronchoalveolar lavage showing a mixture of macrophages and neutrophils. (D) IHC exhibiting inflammatory cells trapped in mucus.

SARS-CoV-2 & Therapies

In a laboratory setting, the SARS-CoV-2 virus was inoculated into human bronchial epithelial cells. This was done in a biosafety level (BSL) 3 laboratory with a MOI of 3. These cells were then examined 96hrs post-infection, using scanning electron microscopy, and recolorized. An en face image (Panel A) shows infected ciliated cells with strand of mucus (yellow) attached to cilia tips (blue). Higher power magnification image (Panel B) shows the structure and density of SARS-CoV-2 virions (red) produced by human airway epithelia. Virus production was approximately 3×106 PFU/ml, consistent with a high number of virions produced and released per cell.

Electron microscopy image showing low magnification of the SARS-CoV-2 virus infecting human bronchial epithelial cells. The image is described in more detail in the text above.
Panel A
Electron microscopy image showing high magnification of the SARS-CoV-2 virus infecting human bronchial epithelial cells. The image is described in more detail in the text above.
Panel B

Selected Bibliography:

  1. Sines B, Morrison CB, Donaldson JM, Ahmad A, Krishnamurthy A, Peden DB, Ehre C. . Ann Am Thorac Soc. 2025 Mar;22(3):339-349. doi: 10.1513/AnnalsATS.202405-507OC. PMID: 39499775. PMCID: PMC11892659.
  2. Brocke SA, Reidel B, Ehre C, Rebuli ME, Robinette C, Schichlein KD, Brooks CA, Jaspers I. . PLoS One. 2024 Dec 31;19(12):e0306197. doi: 10.1371/journal.pone.0306197. PMID: 39739661; PMCID: PMC11687774.
  3. Ehre C, Lopes-Pacheco M, Laselva O. . Front Mol Biosci. 2024 Jun 24;11:1446875. doi: 10.3389/fmolb.2024.1446875. PMID: 38978977; PMCID: PMC11228291.
  4. Meganck RM, Edwards CE, Mallory ML, Lee RE, Dang H, Bailey AB, Wykoff JA, Gallant SC, Zhu DR, Yount BL, Kato T, Shaffer KM, Nakano S, Cawley AM, Sontake V, Wang JR, Hagan RS, Miller MB, Tata PR, Randell SH, Tse LV, Ehre C, Okuda K, Boucher RC, Baric RS. . Cell Rep. 2024 Apr 23;43(4):114076. doi: 10.1016/j.celrep.2024.114076. PMID: 38607917. PMCID: PMC11165423.
  5. Donoghue LJ, Markovetz MR, Morrison CB, Chen G, McFadden KM, Sadritabrizi T, Gutay MI, Kato T, Rogers TD, Snead JY, Livraghi-Butrico A, Button B, Ehre C, Grubb BR, Hill DB, Kelada SNP. . Am J Physiol Lung Cell Mol Physiol. 2023 Dec 1;325(6):L765-L775. doi: 10.1152/ajplung.00390.2022. PMID: 37847709. PMCID: PMC11068428.
  6. Mikami Y, Grubb BR, Rogers TD, Dang H, Asakura T, Kota P, Gilmore RC, Okuda K, Morton LC, Sun L, Chen G, Wykoff JA, Ehre C, Vilar J, van Heusden C, Livraghi-Butrico A, Gentzsch M, Button B, Stutts MJ, Randell SH, O’Neal WK, Boucher RC. . Sci Transl Med. 2023 Jun 7;15(699):eabo7728. doi: 10.1126/scitranslmed.abo7728. PMID: 37285404. PMCID: PMC10664029.
  7. Ehre C, Hansson GC, Thornton DJ, Ostedgaard LS. . J Cyst Fibros. 2023 Mar;22 Suppl 1:S23-S26. doi: 10.1016/j.jcf.2022.08.019. PMID: 36117114. PMCID: PMC10018425.
  8. Kato T, Asakura T, Edwards CE, Dang H, Mikami Y, Okuda K, Chen G, Sun L, Gilmore RC, Hawkins P, De la Cruz G, Cooley MR, Bailey AB, Hewitt SM, Chertow DS, Borczuk AC, Salvatore S, Martinez FJ, Thorne LB, Askin FB, Ehre C, Randell SH, O’Neal WK, Baric RS, Boucher RC; NIH COVID-19 Autopsy Consortium. . Am J Respir Crit Care Med. 2022 Dec 1;206(11):1336-1352. doi: 10.1164/rccm.202111-2606OC. PMID: 35816430. PMCID: PMC9746856.
  9. Hoang ON, Ermund A, Jaramillo AM, Fakih D, French CB, Flores JR, Karmouty-Quintana H, Magnusson JM, Fois G, Fauler M, Frick M, Braubach P, Hales JB, Kurten RC, Panettieri R, Vergara L, Ehre C, Adachi R, Tuvim MJ, Hansson GC, Dickey BF. . Am J Respir Crit Care Med. 2022 Nov 1;206(9):1081-1095. doi: 10.1164/rccm.202202-0309OC. PMID: 35776514. PMCID: PMC9704839.
  10. Markovetz MR, Garbarine IC, Morrison CB, Kissner WJ, Seim I, Forest MG, Papanikolas MJ, Freeman R, Ceppe A, Ghio A, Alexis NE, Stick SM, Ehre C, Boucher RC, Esther CR, Muhlebach MS, Hill DB. . J Cyst Fibros. 2022 Nov;21(6):959-966. doi: 10.1016/j.jcf.2022.04.008. PMID: 35437233.
  11. Okuda K, Shaffer KM, Ehre C. . Int J Mol Sci. 2022 Sep 6;23(18):10232. doi: 10.3390/ijms231810232. PMID: 36142171. PMCID: PMC9499620.
  12. Tse LV, Meganck RM, Araba KC, Yount BL, Shaffer KM, Hou YJ, Munt JE, Adams LE, Wykoff JA, Morowitz JM, Dong S, Magness ST, Marzluff WF, Gonzalez LM, Ehre C, Baric RS. . Proc Natl Acad Sci U S A. 2022 May 3;119(18):e2118126119. doi: 10.1073/pnas.2118126119. PMID: 35476513. PMCID: PMC9170153.
  13. Wykoff JA, Shaffer KM, Araba KC, Markovetz MR, Patarin J, Robert de Saint Vincent M, Donaldson SH, Ehre C. . J Vis Exp. 2022 Apr 21;(182). doi: 10.3791/63876. PMID: 35532240.
  14. Morrison CB, Edwards CE, Shaffer KM, Araba KC, Wykoff JA, Williams DR, Asakura T, Dang H, Morton LC, Gilmore RC, O’Neal WK, Boucher RC, Baric RS, Ehre C. . Proc Natl Acad Sci U S A. 2022 Apr 19;119(16):e2119680119. doi: 10.1073/pnas.2119680119. PMID: 35353667. PMCID: PMC9169748.
  15. Kato T, Radicioni G, Papanikolas MJ, Stoychev GV, Markovetz MR, Aoki K, Porterfield M, Okuda K, Barbosa Cardenas SM, Gilmore RC, Morrison CB, Ehre C, Burns KA, White KK, Brennan TA, Goodell HP, Thacker H, Loznev HT, Forsberg LJ, Nagase T, Rubinstein M, Randell SH, Tiemeyer M, Hill DB, Kesimer M, O’Neal WK, Ballard ST, Freeman R, Button B, Boucher RC. . Sci Adv. 2022 Apr;8(13):eabm9718. doi: 10.1126/sciadv.abm9718. PMID: 35363522.
  16. Morrison CB, Shaffer KM, Araba KC, Markovetz MR, Wykoff JA, Quinney NL, Hao S, Delion MF, Flen AL, Morton LC, Liao J, Hill DB, Drumm ML, O’Neal WK, Kesimer M, Gentzsch M,聽Ehre C.听. Eur Respir J.听2022 Feb 3;59(2):2100185. doi: 10.1183/13993003.00185-2021. PMID: 34172469.听PMCID: PMC8859811.
  17. Howard RL, Markovetz M, Wang Y, Ehre C, Sheikh SZ, Allbritton NL, Hill DB. . Biophys J. 2021 Dec 7;120(23):5384-5394. doi: 10.1016/j.bpj.2021.10.024. PMID: 34695384. PMCID: PMC8715165.
  18. Carpenter J, Wang Y, Gupta R, Li Y, Haridass P, Subramani DB, Reidel B, Morton L, Ridley C, O’Neal WK, Buisine MP, Ehre C, Thornton DJ, Kesimer M. . Proc Natl Acad Sci U S A. 2021 Sep 28;118(39):e2104490118. doi: 10.1073/pnas.2104490118. PMID: 34548396; PMCID: PMC8488587.
  19. Toyonaga T, Araba KC, Kennedy MM, Keith BP, Wolber EA, Beasley C, Steinbach EC, Schaner MR, Jain A, Long MD, Barnes EL, Herfarth HH, Isaacs KL, Hansen JJ, Kapadia MR, Guillem JG, Gulati AS, Sethupathy P, Furey TS, Ehre C, Sheikh SZ. . Sci Rep. 2021 Jun 29;11(1):13533. doi: 10.1038/s41598-021-92979-2. PMID: 34188154; PMCID: PMC8241995.
  20. Escobar YH, Morrison CB, Chen Y, Hickman E, Love CA, Rebuli ME, Surratt JD, Ehre C, Jaspers I. . Am J Physiol Lung Cell Mol Physiol. 2021 Jun 1;320(6):L1064-L1073. doi: 10.1152/ajplung.00525.2020. PMID: 33825493. PMCID: PMC8285624.
  21. Kato T, Mikami Y, Sun L, Rogers TD, Grubb BR, Morrison CB, Ehre C, Sears PR, Ostrowski LE, Randell SH, Boucher RC. . Am J Respir Cell Mol Biol. 2021 Jun;64(6):760-764. doi: 10.1165/rcmb.2021-0033LE. PMID: 33788673. PMCID: PMC8456889.
  22. Hou YJ, Chiba S, Halfmann P, Ehre C, Kuroda M, Dinnon KH 3rd, Leist SR, Sch盲fer A, Nakajima N, Takahashi K, Lee RE, Mascenik TM, Graham R, Edwards CE, Tse LV, Okuda K, Markmann AJ, Bartelt L, de Silva A, Margolis DM, Boucher RC, Randell SH, Suzuki T, Gralinski LE, Kawaoka Y, Baric RS. . Science. 2020 Dec 18;370(6523):1464-1468. doi: 10.1126/science.abe8499. PMID: 33184236. PMCID: PMC7775736.
  23. Ehre C. . N Engl J Med. 2020 Sep 3;383(10):969. doi: 10.1056/NEJMicm2023328. PMID: 32877585. PMCID: PMC7484746.
  24. Danahay H, Fox R, Lilley S, Charlton H, Adley K, Christie L, Ansari E, Ehre C, Flen A, Tuvim MJ, Dickey BF, Williams C, Beaudoin S, Collingwood SP, Gosling M. . FASEB Bioadv. 2020 Jul 1;2(8):464-477. doi: 10.1096/fba.2020-00035. PMID: 32821878; PMCID: PMC7429354.
  25. Ramsey KA, Chen ACH, Radicioni G, Lourie R, Martin M, Broomfield A, Sheng YH, Hasnain SZ, Radford-Smith G, Simms LA, Burr L, Thornton DJ, Bowler SD, Livengood S, Ceppe A, Knowles MR, Noone PG, Donaldson SH, Hill DB, Ehre C, Button B, Alexis NE, Kesimer M, Boucher RC, McGuckin MA. . Am J Respir Crit Care Med. 2020 Mar 15;201(6):661-670. doi: 10.1164/rccm.201906-1219OC. PMID: 31765597. PMCID: PMC7068838.
  26. Lewis BW, Vo T, Choudhary I, Kidder A, Bathula C, Ehre C, Wakamatsu N, Patial S, Saini Y. . J Immunol. 2020 Mar 15;204(6):1650-1660. doi: 10.4049/jimmunol.1900234. PMID: 32060135. PMCID: PMC7714586.
  27. Ehre C. . Med Sci (Paris). 2019 Dec;35(12):1217-1220. doi: 10.1051/medsci/2019234. PMID: 31903945.
  28. Morrison CB, Markovetz MR, Ehre C. . Pediatr Pulmonol. 2019 Nov;54 Suppl 3:S84-S96. doi: 10.1002/ppul.24530. PMID: 31715083. PMCID: PMC6853602.
  29. Markovetz MR, Subramani DB, Kissner WJ, Morrison CB, Garbarine IC, Ghio A, Ramsey KA, Arora H, Kumar PA, Nix DB, Kumagai T, Krunkosky TM, Krause DC, Radicioni G, Alexis NE, Kesimer M, Tiemeyer M, Boucher RC, Ehre C, Hill DB. . Am J Physiol Lung Cell Mol Physiol. 2019 Oct 1;317(4):L498-L509. doi: 10.1152/ajplung.00238.2019. PMID: 31389736. PMCID: PMC6842913.
  30. Esther CR Jr, Muhlebach MS, Ehre C, Hill DB, Wolfgang MC, Kesimer M, Ramsey KA, Markovetz MR, Garbarine IC, Forest MG, Seim I, Zorn B, Morrison CB, Delion MF, Thelin WR, Villalon D, Sabeter JR, Turkovic L, Ranganathan S, Stick SM, Boucher RC, on behalf of AREST CF. . Sci Transl Med. 2019 Apr 3;11(486). doi: 10.1126/scitranslmed.aav3488. PMID: 30944166. PMCID: PMC6566903.
  31. Okuda K,聽Chen G, Subramani DB, Wolf M, Gilmore RC, Kato T, Radicioni G, Kesimer M, Chua M, Dang H, Livraghi-Butrico A, Ehre C, Doerschuk CM, Randell SH, Matsui H, Nagase T, O鈥橬eal WK, Boucher RC. . Am J Respir Crit Care Med. 2019 Mar 15;199(6):715-727. doi: 10.1164/rccm.201804-0734OC. PMID: 30352166. PMCID: PMC6423099.
  32. Ehre C, Rushton ZL, Wang B, Hothem LN, Morrison CB, Fontana NC, Markovetz MR, Delion MF, Kato T, Villalon D, Thelin WR, Esther CR Jr, Hill DB, Grubb BR, Livraghi-Butrico A, Donaldson SH, Boucher RC. . Am J Respir Crit Care Med. 2019 Jan 15;199(2):171-180. doi: 10.1164/rccm.201802-0245OC. PMID: 30212240.听PMCID:聽PMC6353008.
  33. Bauer AK,聽Umer M,聽Richardson VL,聽Cumpian AM,聽Harder AQ,聽Khosravi N,聽Azzegagh Z,聽Hara NM,聽Ehre C,聽Mohebnasab M,聽Caetano MS,聽Merrick DT,聽van Bokhoven A,聽Wistuba II,聽Kadara H,聽Dickey BF,聽Velmurugan K,聽Mann PR,聽Lu X,聽Bar贸n AE,聽Evans CM,聽Moghaddam SJ.听. JCI Insight.听2018 Aug 9;3(15).听doi: 10.1172/jci.insight.120941. PMID:聽30089720. PMCID:聽PMC6129115.
  34. Meng H, Lockshin C, Jain S, Shaligram U, Martinez J, Genkin D, Hill DB, Ehre C, Clark D, Hoppe H. . Recent Pat Drug Deliv Formul. 2018;12(3):212-222. doi: 10.2174/1872211312666180717164758. PMID: 30019653.
  35. Reighard KP*, Ehre C*, Rushton Z, Ahonen M, Hill DB, Schoenfisch M. . ACS Biomater Sci Eng. 2017;3(6):1017鈥�26. doi: 10.1021/acsbiomaterials.7b00039. PMID:聽30320206. PMCID:聽PMC6178828.
  36. Ramsey KA, Rushton ZL, Ehre C. J Vis Exp. 2016 Jun 14;(112). doi: 10.3791/54153. PMID: 27341489. PMCID: PMC4927784.
  37. Ehre C, Ridley C, Thornton DJ. Int J Biochem Cell Biol. 2014 Jul;52:136-45. doi: 10.1016/j.biocel.2014.03.011. Review. PMID: 24685676. PMCID: PMC4449140.
  38. Henderson AG, Ehre C, Button B, Abdullah LH, Cai LH, Leigh MW, DeMaria GC, Matsui H, Donaldson SH, Davis CW, Sheehan JK, Boucher RC, Kesimer M. J Clin Invest. 2014 Jul;124(7):3047-60. doi: 10.1172/JCI73469. PMID: 24892808. PMCID: PMC4072023.
  39. Ehr茅 C. Med Sci (Paris). 2013 Feb;29(2):144-6. doi: 10.1051/medsci/2013292010. French. PMID: 23452599.
  40. Ehre C, Worthington EN, Liesman RM, Grubb BR, Barbier D, O’Neal WK, Sallenave JM, Pickles RJ, Boucher RC. Proc Natl Acad Sci U S A. 2012 Oct 9;109(41):16528-33. doi: 10.1073/pnas.1206552109. Erratum in: Proc Natl Acad Sci U S A. 2014 Apr 15;111(15):5753. PMID: 23012413. PMCID: PMC3478656.
Close-up of Camille Ehre at a microscope